Computational Provenance & Reproducibility Record

RAISINS - R and AI Solutions for INferential Statistics · Online Statistical Analysis Platform for Agricultural Research

Computational Provenance & Reproducibility Record Completely Randomized Design (CRD) · 3.0.0 · DOI 10.5281/zenodo.22055492

Computational Provenance & Reproducibility Record

RAISINS · Completely Randomized Design (CRD) Module

This Computational Provenance Record documents the statistical computing environment, software dependencies, computational provenance, and bibliographic references associated with the RAISINS Completely Randomized Design (CRD) module. It is intended to support computational reproducibility and software transparency. Detailed statistical methodology, mathematical derivations, and user guidance are provided separately in the official module documentation.

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1 Module Metadata

Parameter Specification
Module Completely Randomized Design (CRD)
Module Version 3.0.0
DOI 10.5281/zenodo.22055492
Document Type Computational workflow
Statistical Engine R
R Version 4.5.2
Reproducibility Execution Environment: Posit Connect · GCR · renv-locked

2 Statistical Dependency Manifest

Package Version Repository Core Statistical Functions
stats 4.5.2 Base R lm(), anova(), manova(), p.adjust()
agricolae 1.3-7 CRAN LSD.test(), HSD.test(), duncan.test()
lsr 0.5.2 CRAN etaSquared()
effectsize 1.0.2 CRAN eta_squared()
broom 1.0.13 CRAN tidy()
gtools 3.9.5 CRAN mixedsort(), mixedorder()
dplyr 1.1.4 CRAN group_by(), summarise()
tidyr 1.3.2 CRAN pivot_longer()

3 Statistical Function Registry

Analytical Role Primary Function(s)
One-way linear model stats::lm(y ~ Treatment)
ANOVA table stats::anova(model)
Effect size (Cohen’s f) lsr::etaSquared(model), converted as sqrt(eta2 / (1 - eta2))
Post-hoc mean separation agricolae::LSD.test() (default), agricolae::HSD.test(), agricolae::duncan.test()
P-value adjustment (LSD) agricolae::LSD.test(..., p.adj =); alternatively stats::p.adjust()
Grouping-letter ordering gtools::mixedsort()
Multivariate analysis stats::manova(), broom::tidy(), effectsize::eta_squared()
Treatment means & summaries dplyr::group_by(), dplyr::summarise(), tidyr::pivot_longer()

4 Default Methods & Parameters

Analysis Step / Parameter Default Method / Value
One-way ANOVA
(lm() + anova())
Model y ~ Treatment (single factor, taken as a factor; one model fitted per response variable)
Sums of squares Sequential (Type I). With a single factor this is identical to Type II and Type III
Error mean square (MSE) Residual SS ÷ residual df from the fitted model; reused by every post-hoc test
Effect size
(lsr::etaSquared())
Treatment effect size Cohen's f, computed from η² as sqrt(eta2 / (1 - eta2))
Significance & rounding Significance level (α) 0.05 default (user-selectable: 0.05, 0.01)
Significance stars ** for p ≤ 0.01, * for p ≤ 0.05, NS otherwise (fixed thresholds, independent of the selected α)
Rounding 2 decimal places default (user-adjustable digit control, 1–10)
Mean comparison / post-hoc
(agricolae)
Default method LSD - agricolae::LSD.test() (alternatives: Tukey HSD HSD.test(); DMRT duncan.test())
Inputs to the test Pooled MSerror = MSE and DFerror = residual df from the one-way model; alpha = α
Grouping display Letters shown only when the treatment p ≤ α; group labels ordered with gtools::mixedsort(); grouping is suppressed when the number of treatments exceeds 80
Critical value reported CD/LSD (LSD), HSD (Tukey), or Duncan critical ranges (DMRT). The trait-wise Individual ANOVA table reports CD at both 5% and 1%
P-value adjustment
(LSD only, LSD.test(p.adj =))
Method none default (user-selectable: bonferroni, holm, BH)
Reporting under adjustment A single CD/critical difference is not defined once comparisons are adjusted, so the CD row is omitted; the adjusted result is carried by the grouping letters
Standard errors SEM sqrt(MSE / r) (balanced replication only; reported as “-” when replication is unequal)
SED sqrt(2 × MSE / r) (balanced replication only)
Data transformations
(optional, per variable)
Logarithmic log10(x); if any value ≤ 0, log10(x - min(x) + 1)
Square-root sqrt(x); if any value = 0, sqrt(x + 0.5); blocked on negative values
Arcsine asin(sqrt(x)) on proportions in [0, 1]; 0 and 1 replaced by 1/(4n) and 1 - 1/(4n)
MANOVA
(multi-response mode)
Test statistic Pillai's trace (the stats::manova() default), tidied with broom::tidy()
Requirement At least two response variables, and the number of treatments must exceed the number of response variables

5 R Code for Key Analytical Steps

The code blocks below demonstrate the exact computation behind each reported result using PlantGrowth (built into the datasets package): a balanced completely randomized experiment with one factor group (control plus two treatments), 10 replicates per treatment, and dried plant weight as the response.

5.1 One-Way ANOVA and Effect Size

data(PlantGrowth)
d <- PlantGrowth                                 # weight ~ group, 3 treatments x 10 reps, balanced

treatment <- factor(d$group)                     # single treatment factor
y         <- d$weight                            # response variable

# One-way linear model and ANOVA table (mirrors the module; one model per response)
model  <- lm(y ~ treatment)
result <- anova(model)
result                                           # F = 4.85, p = 0.0159

# Error (residual) mean square and df, reused by the post-hoc tests
MSE   <- result[2, 3]                            # 0.3886
dferr <- result[2, 1]                            # 27

# Cohen's f effect size, derived from eta-squared
eta2 <- lsr::etaSquared(model)[1, 1]             # 0.2641
sqrt(eta2 / (1 - eta2))                          # 0.5991

5.2 Post-hoc Mean Comparison (LSD default; Tukey / DMRT alternatives)

alpha <- 0.05                                    # default significance level

# Least Significant Difference (default): agricolae uses the pooled MSE and
# residual df from the one-way model; grouping letters ordered with mixedsort.
out <- agricolae::LSD.test(y, treatment, DFerror = dferr, MSerror = MSE,
                           alpha = alpha, p.adj = "none", group = TRUE)

out$groups[gtools::mixedsort(rownames(out$groups)), ]
out$statistics                                   # MSerror, Df, Mean, CV, t.value, LSD (= CD)

# Grouping letters are printed only when the treatment effect is significant:
if (result[1, 5] > alpha) out$groups[, 2] <- ""

# Optional p-value adjustment for LSD (default "none"; selectable: bonferroni, holm, BH).
# agricolae applies the correction internally and recomputes the grouping letters.
agricolae::LSD.test(y, treatment, DFerror = dferr, MSerror = MSE, alpha = alpha,
                    p.adj = "bonferroni")$groups

# Alternatives selectable in the app (same DFerror / MSerror / alpha arguments):
#   Tukey HSD -> agricolae::HSD.test(y, treatment, DFerror = dferr, MSerror = MSE, alpha = alpha)
#   DMRT      -> agricolae::duncan.test(y, treatment, DFerror = dferr, MSerror = MSE, alpha = alpha)

# Standard errors from the pooled MSE (r = replications per treatment)
r   <- out$means[1, 3]                           # 10
SEM <- sqrt(MSE / r)                             # 0.1971
SED <- sqrt(2 * MSE / r)                         # 0.2788

5.3 MANOVA Across Multiple Responses (multi-response mode)

# The app's MANOVA mode requires >= 2 response columns (one per measured trait)
# and more treatments than response variables. Illustrated with `iris`, a
# completely randomized layout of 3 treatments x 50 units and 4 measured traits.
data(iris)
trt <- factor(iris$Species)
Y   <- as.matrix(iris[, 1:4])

res.man <- manova(Y ~ trt)
broom::tidy(res.man)                             # Pillai's trace for the treatment term
effectsize::eta_squared(res.man)                 # partial eta-squared

Explore the entire Completely Randomized Design (CRD) module in preview mode using our demo datasets. To submit suggestions or report a workflow issue, please use the discussion section below, or visit the official RAISINS website.

6 RAISINS Native Statistical Framework

RAISINS uses R for all its statistical computations. Every package used to generate major results is listed and demonstrated with examples, so results can be reproduced independently. These results are then organized and formatted on the RAISINS website along with visualisation to make them easier to use and interpret. RAISINS also has its own custom-built statistical tools for managing workflows, validating results, and generating reports. Details of these are not fully covered here, they’re shared with outside researchers only on request, and are subject to licensing terms.

7 Package References

R Core Team. (2025). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/

de Mendiburu, F. (2023). agricolae: Statistical Procedures for Agricultural Research (R package version 1.3-7). https://doi.org/10.32614/CRAN.package.agricolae

Navarro, D. J. (2015). Learning Statistics with R: A Tutorial for Psychology Students and Other Beginners. University of New South Wales. (lsr R package version 0.5.2). https://doi.org/10.32614/CRAN.package.lsr

Ben-Shachar, M. S., Lüdecke, D., & Makowski, D. (2020). effectsize: Estimation of Effect Size Indices and Standardized Parameters (R package version 1.0.2). https://doi.org/10.32614/CRAN.package.effectsize

Robinson, D., Hayes, A., & Couch, S. (2025). broom: Convert Statistical Objects into Tidy Tibbles (R package version 1.0.13). https://doi.org/10.32614/CRAN.package.broom

Warnes, G. R., Bolker, B., & Lumley, T. (2023). gtools: Various R Programming Tools (R package version 3.9.5). https://doi.org/10.32614/CRAN.package.gtools

Wickham, H., François, R., Henry, L., Müller, K., & Vaughan, D. (2023). dplyr: A Grammar of Data Manipulation (R package version 1.1.4). https://doi.org/10.32614/CRAN.package.dplyr

Wickham, H., Vaughan, D., & Girlich, M. (2025). tidyr: Tidy Messy Data (R package version 1.3.2). https://doi.org/10.32614/CRAN.package.tidyr

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